치주인대세포에서 Nicotine과 LPS에 의해 유도된 Human beta Defensin-2의 발현에 MAP kinase의 관여
Involvement of MAP Kinase on Nicotine- and LPS-induced Human Beta Defensin-2 Expression in Periodontal Ligament Cells
이상임, 이화정, 배원정, 강순일, 이소연, 이영만, 신경섭, 박재국, 류원국, 채종문, 김은철,
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이상임 ( Lee Sang-Im ) - 경희대학교 치의학전문대학원 악안면재생학교실
이화정 ( Lee Hwa-Jeong ) - 경희대학교 치의학전문대학원 악안면재생학교실
배원정 ( Bae Won-Jung ) - 경희대학교 치의학전문대학원 악안면재생학교실
강순일 ( Kang Sun-Il ) - 경희대학교 치의학전문대학원 악안면재생학교실
이소연 ( Lee So-Yun ) - 경희대학교 치의학전문대학원 악안면재생학교실
이영만 ( Lee Young-Man ) - 경희대학교 치의학전문대학원 악안면재생학교실
신경섭 ( Shin Kyung-Sub ) - 원광대학교 치과대학 구강악안면병리학교실
박재국 ( Park Jae-Kook ) - 원광대학교 치과대학 치과교정학교실
류원국 ( Ryu Won-Kook ) - 원광대학교 치과대학 치과교정학교실
채종문 ( Chae Jong-Moon ) - 원광대학교 치과대학 치과교정학교실
김은철 ( Kim Eun-Cheol ) - 경희대학교 치의학전문대학원 악안면재생학교실
KMID : 0829220110350030161
Abstract
Human β- defensin-2 (hBD-2) is an antimicrobial peptide which is produced by epithelial cells after stimulation with microorganisms or inflammatory mediators. However, little is known as to whether the LPS and nicotine induces the expression of hBD-2 in periodontal l igament (PDL) cells. T he p urpose o f this s tudy was t o investigate t he r oles o f MAPKs pathway o f nicotine a nd L PS-induced hBD-2 expression in PDL cells. The maximal expression of hBD-2 was observed in nicotine 5 mM and LPS 1 ㎍/㎖ treated PDL cells. Nicotine and LPS induced the phosphorylation of p38, c-Jun N-terminal kinase 1 and 2 (JNK1/2), and extracellular signal-regulated kinases 1 and 2 (ERK1/2) MAPK. ERK1/2 inhibitor SB203580, p38 inhibitor PD98059, and JNK inhibitor SP600125, blocked the effects of nicotine and LPS on hBD-2 upregulation in PDL cells. These results collectively suggest that hBD-2 is up-regulated in nicotine and LPS-treated PDL cells through activation of p38, ERK and JNK MAPKs pathway. Our data regarding the up-regulation of hBD-2 may help us to understand the antimicrobial mechanism in periodontal disease.
키워드
hBD-2; LPS; Nicotine; PDL cells
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